A novel method for three-dimensional observation of the vascular networks in the whole mouse brain.

@article{Hashimoto2008ANM,
  title={A novel method for three-dimensional observation of the vascular networks in the whole mouse brain.},
  author={Hisashi Hashimoto and Moriaki Kusakabe and Hiroshi Ishikawa},
  journal={Microscopy research and technique},
  year={2008},
  volume={71 1},
  pages={
          51-9
        }
}
A novel method for acquiring serial images suitable for three-dimensional reconstruction of vascular networks in the whole brain of mouse was developed. The brain infused with a White India ink-gelatin solution was fixed and embedded in paraffin containing Sudan Black B through xylene also containing Sudan Black B. Each sliced surface of the paraffin block was coated with liquid paraffin and its image was serially acquired. Coating with liquid paraffin extremely improved the quality of the… CONTINUE READING