A novel method for the isolation of skin resident T cells from normal and diseased human skin.

@article{Clark2006ANM,
  title={A novel method for the isolation of skin resident T cells from normal and diseased human skin.},
  author={Rachael A Clark and Benjamin F. Chong and Nina Mirchandani and Kei-ichi Yamanaka and George Murphy and Rebecca K. Dowgiert and Thomas S Kupper},
  journal={The Journal of investigative dermatology},
  year={2006},
  volume={126 5},
  pages={1059-70}
}
T cells resident in normal skin likely conduct immunosurveillance and are implicated in the development of inflammatory disorders such as psoriasis. This population of cells is difficult to study because existing techniques allow isolation of only few cells. We report here a novel method of isolating T cells from both normal and diseased human skin. Explants of skin cultured on three-dimensional matrices led to the outgrowth of dermal fibroblasts that elaborated T cell chemoattractant factors… CONTINUE READING