A novel method for selective isotope labeling of bacterially expressed proteins.

@article{Lee1995ANM,
  title={A novel method for selective isotope labeling of bacterially expressed proteins.},
  author={Kyoung Mi Lee and Elliot J Androphy and James D. Baleja},
  journal={Journal of biomolecular NMR},
  year={1995},
  volume={5 1},
  pages={93-6}
}
A novel method for isotope labeling in selected amino acids is presented for use with the T7 RNA polymerase system. The protocol is illustrated with the DNA-binding domain from the E2 protein of bovine papillomavirus, BPV-1. On addition of rifampicin, protein expression occurs exclusively from the gene controlled by the T7 promoter. Since the bacteria are now dedicated to the production of E2 protein, labeling with specific amino acids is efficiently performed. For example, 10 mg/l of 15N… CONTINUE READING

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