A novel method for production of lipid hydroperoxide- or oxysterol-rich low-density lipoprotein.

@article{Gerry2008ANM,
  title={A novel method for production of lipid hydroperoxide- or oxysterol-rich low-density lipoprotein.},
  author={Andrew Gerry and Leanne Satchell and David S. Leake},
  journal={Atherosclerosis},
  year={2008},
  volume={197 2},
  pages={579-87}
}
LDL oxidation may be important in atherosclerosis. Extensive oxidation of LDL by copper induces increased uptake by macrophages, but results in decomposition of hydroperoxides, making it more difficult to investigate the effects of hydroperoxides in oxidised LDL on cell function. We describe here a simple method of oxidising LDL by dialysis against copper ions at 4 degrees C, which inhibits the decomposition of hydroperoxides, and allows the production of LDL rich in hydroperoxides (626+/-98… CONTINUE READING