A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface

Abstract

The Open University's repository of research publications and other research outputs A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface Journal Article (2014). A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface. Biomaterials Science, 2(1) pp. 41–51. For guidance on citations see FAQs. Copyright and Moral Rights for the articles on this site are retained by the individual authors and/or other copyright owners. For more information on Open Research Online's data policy on reuse of materials please consult the policies page. A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface a The capacity to study the deposition of mineral within a hydrogel structure is of significant interest to a range of therapies that seek to replace the hard tissues and the hard–soft tissue interface. Here, a method is presented that utilises Confocal Raman microscopy as a tool for monitoring mineralisation within hydrogels. Synthetic hard–soft material interfaces were fabricated by apposing brushite (a sparingly soluble calcium phosphate) and biopolymer gel monoliths. The resulting structures were matured over a period of 28 days in phosphate buffered saline. Confocal Raman microscopy of the interfacial region showed the appearance of calcium phosphate salt deposits away from the original interface within the biopolymeric structures. Furthermore, the appearance of octacalcium phosphate and carbonated hydro-xyapatite was observed in the region of the brushite cement opposing the biopolymer gel. This study describes not only a method for analysing these composite structures, but also suggests a method for recapitulating the graduated tissue structures that are often found in vivo.

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@inproceedings{Paxton2016ANM, title={A novel method for monitoring mineralisation in hydrogels at the engineered hard–soft tissue interface}, author={Jennifer Z. Paxton and Susanne Koburger and Alistair Bannerman and Liam M Grover and Frank M{\"{u}ller and James Bowen}, year={2016} }