A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates.

@article{Miller1993ANM,
  title={A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates.},
  author={Nicholas J. Miller and Catherine A. Rice-Evans and Michael J Davies and V Gopinathan and Anthony David Milner},
  journal={Clinical science},
  year={1993},
  volume={84 4},
  pages={
          407-12
        }
}
1. A new method has been developed for measuring the total antioxidant capacity of body fluids and drug solutions, based on the absorbance of the ABTS.+ radical cation. 2. An automated method for use on a centrifugal analyser, as well as a manual method, is described. 3. The procedure has been applied to physiological antioxidant compounds and radical-scavenging drugs, and an antioxidant ranking was established based on their reactivity relative to a 1.0 mmol/l Trolox standard. 4. The Trolox… 
View on PubMed
clinsci.org
2,853 Citations
Highly Influential Citations
112
Background Citations
345
Methods Citations
631
Results Citations
9

Figures and Tables from this paper

2,853 Citations

A new automated method for the determination of the Total Antioxidant Capacity (TAC) of human plasma, based on the crocin bleaching assay

An automated assay to calculate the antioxidant capacity of plasma after a subtraction of all interference deriving from endogenous and/or exogenous metabolites is presented, which can be used as a useful indicator of the antioxidant value of foods and beverages in the daily diet.

Spectrophotometric assays for total antioxidant capacity (TAC) in dog serum: an update

It is concluded that, ideally, various different assays integrated in a panel should be used for TAC evaluation, since depending on the assay performed TAC results can be markedly different.

The main and modified CUPRAC methods of antioxidant measurement

A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation.

  • O. Erel
  • Medicine
    Clinical biochemistry
  • 2004

Pitfalls in a method for assessment of total antioxidant capacity.

Results obtained in the ABTS radical-based methods should be reviewed critically before the antioxidant capacity can be assessed, due to both a scavenger effect and an effect on the rate of ABTS oxidation.

An improved, rapid in vitro method to measure antioxidant activity. Application On selected flavonoids and apple juice.

A rapid in vitro method for measuring antioxidant activity is presented, which enables the evaluation of health claims and the optimization of product development with respect to health protecting…

A Novel Assay for Measuring Total Antioxidant Capacity in Whole Blood and Other Biological Samples

A new method for rapidly determining total antioxidant capacity (TAC) in a biological sample has been devised and evaluated and it requires the minimum volumes and time.

Flow injection based methods for fast screening of antioxidant capacity.

In vivo total antioxidant capacity: comparison of different analytical methods.

Non-enzymatic antioxidant capacity assays: Limitations of use in biomedicine

This mini-review shows shortcomings of TAC assays and points of concern that should be considered when performing and interpreting results of such assays.
...

References

SHOWING 1-10 OF 29 REFERENCES

Serum antioxidant activity in premature babies.

Lipid-peroxidation of bovine-brain homogenate could be inhibited in vitro by the addition of exogen antioxidant, and in this assay MTDQ-DS, Cavinton, DPA-HC1, vitamin E, A, C showed different antioxidant activity.

The Total Free Radical Trapping Ability of Cord Blood Plasma in Preterm and Term Babies

The efficient plasma radical trapping capacity in the cord blood may partly compensate for deficiencies in other components of the antioxidant defenses, e.g. cellular enzymes, at the time of birth.

Assay using brain homogenate for measuring the antioxidant activity of biological fluids.

Fresh ox-brain homogenate prepared under standard conditions at 4°C and stored at - 20°C autoxidizes spontaneously and reproducibly when re-heated to 37°C, and can be used for assaying the antioxidant activity of serum and of other biological fluids.

[19] Uric acid: Functions and determination

The relative contributions of vitamin E, urate, ascorbate and proteins to the total peroxyl radical-trapping antioxidant activity of human blood plasma.

The enzymatic oxidation of Desferal to a nitroxide free radical

Antioxidant defenses and lipid peroxidation in human blood plasma.

The data suggest that in states of leukocyte activation and other types of acute or chronic oxidative stress such a simple regimen as controlled ascorbate supplementation could prove helpful in preventing formation of lipid hydroperoxides, some of which cannot be detoxified by endogenous plasma activities and thus might cause damage to critical targets.

On the mechanism of peroxidation of uric acids by hemoproteins.

The formation of free radicals by cardiac myocytes under oxidative stress and the effects of electron-donating drugs.

Biochemical investigations of the potential for myoglobin in ruptured myocytes to be involved in radical generation, when under oxidative stress, and of the nature of the resulting species revealed a radical species which is capable of inducing membrane lipid peroxidation.

Prevention of bronchopulmonary dysplasia with vitamin E administration during the acute stages of respiratory distress syndrome.