A novel method for evaluation of capillarity in human skeletal muscles from confocal 3D images.

@article{Janacek2011ANM,
  title={A novel method for evaluation of capillarity in human skeletal muscles from confocal 3D images.},
  author={Jiri Janacek and Erika Cvetko and Lucie Kub{\'i}nov{\'a} and Ludvik Travnik and Ida Er{\vz}en},
  journal={Microvascular research},
  year={2011},
  volume={81 2},
  pages={231-8}
}
A well developed capillary bed is essential for proper function of skeletal muscles. We present for the first time a triple immunofluorescent method suitable for staining capillaries and muscle fibre outlines in thick sections of human skeletal muscle, applying antibodies against collagen IV (in red) and F8 (in green) as well as Ulex europaeus lectin, visualized in green fluorescence. Further, we present possibilities for quantitative evaluation of the capillary network which implies the length… CONTINUE READING
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