A novel medium for the isolation of N-acylhomoserine lactone-degrading bacteria

  title={A novel medium for the isolation of N-acylhomoserine lactone-degrading bacteria},
  author={Kok-Gan Chan and Wai-Fong Yin and Choon-Kook Sam and C. Koh},
  journal={Journal of Industrial Microbiology \& Biotechnology},
A novel chemically defined medium, named KG medium, supplemented with N-3-oxo-hexanoylhomoserine lactone (3-oxo-C6-HSL), an acylhomoserine lactone (AHL) used as signalling molecules in Gram-negative bacterial cell-to-cell communication, as the sole source of carbon and nitrogen, was designed and successfully used for the enrichment and isolation of AHL-degrading bacteria. A 3-oxo-C6-HSL-degrading bacterium, 13sw7, was isolated from sewage after six enrichment transfers in the 3-oxo-C6-HSL… Expand
Rapid degradation of N-3-oxo-acylhomoserine lactones by a Bacilluscereus isolate from Malaysian rainforest soil
A bacterial strain, KM1S, was isolated from a Malaysian rainforest soil sample by using a defined enrichment medium that specifically facilitates selection of quorum quenching bacteria and indicated the presence of the motif 106HXDH-59 amino acids-H169-21 amino acid-D191 for N-acylhomoserine lactone lactonases. Expand
Isolation and identification of quorum quenching bacteria from environmental samples.
A high-throughput method for the isolation and identification of AHL-degrading bacteria from environmental samples is developed and it is likely that some isolates produce and secrete a heat-stable, low molecular weight inhibitory compound(s). Expand
Coexistence of quorum-quenching and quorum-sensing in tropical marine Pseudomonas aeruginosa strain MW3A
A novel marine P. aeruginosa strain MW3A possessing both quorum-quenching and quorum/quorum-sensing properties is reported here. Expand
AidP, a novel N-Acyl homoserine lactonase gene from Antarctic Planococcus sp.
This study demonstrated the QQ activity of a psychrotolerant strain of Planococcus versutus strain L10.15T, isolated from a soil sample obtained near an elephant seal wallow in Antarctica, and suggested that it is a novel and uncharacterized class of AHL lactonase. Expand
Short Chain N-acyl Homoserine Lactone Production by Soil Isolate Burkholderia sp. Strain A9
The present study aimed to characterize the production of AHL of a bacterial strain A9 isolated from a Malaysian tropical soil using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry and 16S rDNA nucleotide sequence analysis. Expand
Selection of the N-Acylhomoserine Lactone-Degrading Bacterium Alteromonas stellipolaris PQQ-42 and of Its Potential for Biocontrol in Aquaculture
The screening of QQ activity amongst 450 strains isolated from a bivalve hatchery in Granada (Spain), and the selection of the strain PQQ-42, which degrades a wide range of N-acylhomoserine lactones (AHLs), suggest that this AHL-degrading bacterium may have biotechnological applications in aquaculture. Expand
Stenotrophomonas maltophilia AHL-Degrading Strains Isolated from Marine Invertebrate Microbiota Attenuate the Virulence of Pectobacterium carotovorum and Vibrio coralliilyticus
The evidence of marine organisms as an underexplored and promising source of QQ enzymes, useful to prevent infections in aquaculture and agriculture is strengthened. Expand
Labrenzia sp. BM1: A Quorum Quenching Bacterium That Degrades N-acyl Homoserine Lactones via Lactonase Activity
To the best of the knowledge, this is the first documentation of a Labrenzia sp. Expand
AhaP, A Quorum Quenching Acylase from Psychrobacter sp. M9-54-1 That Attenuates Pseudomonas aeruginosa and Vibrio coralliilyticus Virulence
The whole genome sequence of this strain was determined and it was found that the full 16S rRNA sequence shares 99.78–99.66% identity with Psychrobacter pulmonis CECT 5989T and P. faecalis ISO-46T. Expand
Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia
The data show that ginger rhizosphere bacteria which make and degrade a wide range of AHLs are likely to play a collective role in determining the QS-dependent phenotype of a polymicrobial community. Expand


Metabolism of Acyl-Homoserine Lactone Quorum-Sensing Signals by Variovorax paradoxus
This study indicates that some strains of V. paradoxus degrade and grow on acyl-HSL signals as the sole energy and nitrogen sources. Expand
N-Acylhomoserine Lactones Undergo Lactonolysis in a pH-, Temperature-, and Acyl Chain Length-Dependent Manner during Growth of Yersinia pseudotuberculosis and Pseudomonas aeruginosa
Data show that to be functional under physiological conditions in mammalian tissue fluids, AHLs require an N-acyl side chain of at least four carbons in length and that the longer the acyl side chain the more stable the AHL signal molecule. Expand
Novel bacteria degrading N-acylhomoserine lactones and their use as quenchers of quorum-sensing-regulated functions of plant-pathogenic bacteria.
The diversity of the QS-interfering bacteria in the rhizosphere is revealed and the validity of targeting QS signal molecules to control pathogens with natural bacterial isolates is demonstrated. Expand
Quorum Quenching by an N-Acyl-Homoserine Lactone Acylase from Pseudomonas aeruginosa PAO1
The quorum-quenching AHL acylase may enable P. aeruginosa PAO1 to modulate its own quorum -sensing-dependent pathogenic potential and, moreover, offers possibilities for novel antipseudomonal therapies. Expand
Quorum sensing and Chromobacterium violaceum: exploitation of violacein production and inhibition for the detection of N-acylhomoserine lactones.
The ability of CV026 to respond to a series of synthetic AHL and N-acylhomocysteine thiolactone (AHT) analogues is explored, greatly extending the ability to detect a wide spectrum of AHL signal molecules. Expand
Rapid isolation method of soil bacilli and screening of their quorum quenching activity
In Gram-negative bacteria, quorum sensing is mediated by N-acylhomoserine lactones (AHL) to regulate different biological functions, including production of virulence factors. Quorum quenching refersExpand
AiiA, an enzyme that inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora.
The results indicate that the AI-inactivation approach represents a promising strategy for prevention of diseases in which virulence is regulated by AIs. Expand
Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase
The acyl-homoserine lactonase (AHL-lactonase), a new enzyme from Bacillus sp. Expand
Phylogeny of the Defined Murine Microbiota: Altered Schaedler Flora
  • F. Dewhirst, Chih-Ching Chien, +4 authors J. Fox
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • 1999
The 16S rRNA sequence information determined in the present study should allow rapid identification of ASF strains and should permit detailed analysis of the interactions of AsF organisms during development of intestinal disease in mice that are coinfected with a variety of pathogenic microorganisms. Expand
The bacterial cell wall as a source of antibacterial targets
  • D. Green
  • Biology, Medicine
  • Expert opinion on therapeutic targets
  • 2002
This review focuses on target-based approaches for developing new chemical classes of antibacterial agents aimed at the bacterial cell wall and the influence of bacterial genetics and genomics, structural biology, assay development and the properties of known inhibitors on these approaches will be discussed in the context of drug discovery. Expand