A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.

@article{Masayama2007ANL,
  title={A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of Bacillus subtilis, is involved in spore germination.},
  author={Atsushi Masayama and Ritsuko Kuwana and Hiromu Takamatsu and Hisashi Hemmi and Tohru Yoshimura and Kazuhito Watabe and Ryuichi Moriyama},
  journal={Journal of bacteriology},
  year={2007},
  volume={189 6},
  pages={2369-75}
}
The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-green fluorescent protein fusion protein produced in sporulating cells was detectable in the mother cell but not in the forespore compartment under fluorescence microscopy, and the… CONTINUE READING