A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from cicada (Cicada flammata)

Abstract

A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from dried cicadas. It was adsorbed on Q-Sepharose and unadsorbed on Affi-Gel Blue gel. Its hemagglutinating activity was stable up to 55 °C and between pH 2 and 13. The activity was inhibited by glucuronic acid and raffinose, K+ ions, and Mg2+ ions. Cicada lectin potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.76 and 0.49 µM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.36 μM but was devoid of mitogenic activity on spleen cells. Its N-terminal sequence exhibited slight similarity to a conserved hypothetical protein from Culex quinquefasciatus and a gene product from transcript GH19834-RA of Drosophila grimshawi, but there was no resemblance to lectins from other insects, including Drosophila, Sarcophaga, Glossina, and Aedes species.

DOI: 10.1007/s00253-009-2363-3

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Cite this paper

@article{Ye2009ANL, title={A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from cicada (Cicada flammata)}, author={Xiu juan Ye and Tzi Bun Ng}, journal={Applied Microbiology and Biotechnology}, year={2009}, volume={86}, pages={1409-1418} }