A novel lectin-based enzyme-linked immunosorbent assay for the measurement of IgA1 in serum and secretory IgA1 in secretions.

@article{Cullina1993ANL,
  title={A novel lectin-based enzyme-linked immunosorbent assay for the measurement of IgA1 in serum and secretory IgA1 in secretions.},
  author={M J Cullina and J. F. Greally},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1993},
  volume={216 1-2},
  pages={
          23-38
        }
}
Jackfruit lectin (Jacalin) specifically binds IgA1 and secretory IgA1 (sIgA1). Jacalin was successfully purified, and used as the immobilized phase in novel noncompetitive enzyme-linked immunosorbent assays (ELISAs) for the measurement of IgA1 in serum and sIgA1 in saliva, using jacalin affinity purified material as standards. A non-competitive ELISA for the measurement of total sIgA was also developed using commercially available reagents.