A novel label-free optical cysteine sensor based on the competitive oxidation reaction catalyzed by G-quadruplex halves.

Abstract

A sensitive and selective colorimetric detection method for Cysteine (Cys) was established in this paper. The detection mechanism is based on the oxidation of Cys by H2O2, which prevents the catalysis of the 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-H2O2 reaction by G-quadruplex halves. With the addition of Cys, the amount of the blue… (More)
DOI: 10.1016/j.bios.2012.12.032

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