A novel inserted membrane technique for studies of mycorrhizal extraradical mycelium

@article{Balaz2001ANI,
  title={A novel inserted membrane technique for studies of mycorrhizal extraradical mycelium},
  author={Milan Balaz and Miroslav Vos{\'a}tka},
  journal={Mycorrhiza},
  year={2001},
  volume={11},
  pages={291-296}
}
A simple "inserted membrane technique" (IMT) for sampling mycorrhizal extraradical mycelium (ERM) was developed as an alternative to the commonly used membrane filtration technique (MFT). The ERM of two types of mycorrhiza, orchid and arbuscular, was extracted by insertion of cellulose nitrate or cellulose acetate membrane filters (0.45–0.6 µm pore size) into the mycorrhizosphere of host plants. The membranes with adhered ERM were removed at harvest and stained with trypan blue for estimation… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 25 references

Compar - ison of techniques for extraction and quantification of extraradical mycelium of arbuscular mycorrhizal fungi in soils

  • CL Boddington, EE Bassett, I Jakobsen, JC Dodd
  • Soil Biol Biochem
  • 1999

Protocol for assessing the viability of Sphaerotheca fuliginea conidia by tetrazolium chloride ( INT ) reduction

  • IM Weiersbye-Witkowski, CJ Straker
  • S Afr J Bot
  • 1997

Approaches to the study of extraradical mycelium of arbuscular mycorrhizal fungi

  • JC Dodd
  • Impact of arbuscular mycorrhizas on sustainable…
  • 1994

Recognition and infection process , basis for host specificity of arbuscular mycorrhizal fungi

  • M Giovannetti, C Sbrana, +4 authors S Gianinazzi
  • 1994

Extraction of vesiculararbuscular mycorrhizal mycelium from sand samples

  • A Vilariño, J Arines, H Schüepp
  • Soil Biol Biochem
  • 1993

In planta histochemical staining of fungal alkaline phosphatase activity for analysis of efficient arbuscular mycorrhizal infections

  • Norris, DJ Read, +4 authors A Gollotte
  • Mycol Res
  • 1993

Establishment of vesiculararbuscular mycorrhiza in root organ culture : review and proposed methodology

  • G Bécard, Y Piché
  • 1992

External hyphae of vesiculararbuscular mycorrhizal fungi associated with Trifolium subterraneum L . 2 . Hyphal transport of 32 P over defined distances

  • I Jakobsen, LK Abbott, AD Robson
  • New Phytol
  • 1992

Distribution , structure , and function of external hyphae of vesiculararbuscular mycorrhizal fungi

  • DM Sylvia
  • 1990

Similar Papers

Loading similar papers…