A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments.

@article{Thorn2009ANI,
  title={A novel in vitro flat-bed perfusion biofilm model for determining the potential antimicrobial efficacy of topical wound treatments.},
  author={Robin Michael Statham Thorn and John Greenman},
  journal={Journal of applied microbiology},
  year={2009},
  volume={107 6},
  pages={2070-9}
}
AIMS To develop an in vitro flat-bed perfusion biofilm model that could be used to determine the antimicrobial efficacy of topically applied treatments. METHODS AND RESULTS Pseudomonas aeruginosa and Staphylococcus aureus biofilms were grown within continuously perfused cellulose matrices. Enumeration of the biofilm density and eluate was performed at various sampling times, enabling determination of the biofilm growth rate. Two antimicrobial wound dressings were applied to the surface of… CONTINUE READING