A novel in vitro device to deliver induced electromagnetic fields to cell and tissue cultures.

@article{Ravin2020ANI,
  title={A novel in vitro device to deliver induced electromagnetic fields to cell and tissue cultures.},
  author={Rea Ravin and Teddy X. Cai and Randall Pursley and Marcial Garmendia-Cedillos and Thomas J. Pohida and Raisa Z. Freidlin and Herui Wang and Zhengping Zhuang and Amber J. Giles and Nathan H. Williamson and Mark R. Gilbert and Peter J. Basser},
  journal={Biophysical journal},
  year={2020}
}

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References

SHOWING 1-10 OF 53 REFERENCES

An Overview of Sub-Cellular Mechanisms Involved in the Action of TTFields

TLDR
An assessment of possible physical interactions between 100 kHz range alternating electric fields and biological cells in general and their nano-scale subcellular structures in particular concludes that the most likely candidates to provide a quantitative explanation of these effects are ionic condensation waves around microtubules as well as dielectrophoretic effects on the dipole moments of microtubule.

Alternating Electric Fields (TTFields) Activate Cav1.2 Channels in Human Glioblastoma Cells

TLDR
The present study identified Cav1.2 channels as TTFields target in the plasma membrane and provides the rationale to combine T TFields therapy with Ca2+ antagonists that are already in clinical use.

Modeling Tumor Treating Fields (TTFields) application in single cells during metaphase and telophase

TLDR
A computational framework is developed to investigate the mechanisms of action of these Tumor Treating Fields and to understand in vitro findings observed in cell culture, and it is shown that peak dielectrophoretic forces develop within dividing cells exposed to TTFields.

Determining the Optimal Inhibitory Frequency for Cancerous Cells Using Tumor Treating Fields (TTFields)

TLDR
The results presented in this report demonstrate that the optimal frequency of the TTFields with respect to both cell counts and clonogenic assays is 200 kHz for both ovarian and glioma cells.

The electric field distribution in the brain during TTFields therapy and its dependence on tissue dielectric properties and anatomy: a computational study.

TLDR
This modelling study predicts that during treatment with TTFields the electric field in the tumour exceeds 1 V cm−1, independent of modelling assumptions.

How a High-Gradient Magnetic Field Could Affect Cell Life

TLDR
It is found that a relatively small magnetic field with a large gradient can significantly change the membrane potential of the cell and thus have a significant impact on not only the properties and biological functionality of cells but also cell fate.

A Review on Tumor-Treating Fields (TTFields): Clinical Implications Inferred From Computational Modeling

TLDR
Computational approaches used to characterize TTFields are reviewed, outlining the potential clinical value inferred from computational modeling in the human head and of the microscopic field distribution in tumor cells.

Disruption of cancer cell replication by alternating electric fields.

TLDR
In vivo treatment of tumors in C57BL/6 and BALB/c mice resulted in significant slowing of tumor growth and extensive destruction of tumor cells within 3-6 days, demonstrating the potential applicability of the described electric fields as a novel therapeutic modality for malignant tumors.

Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors

TLDR
TTFields are a safe and effective new treatment modality which effectively slows down tumor growth in vitro, in vivo and, as demonstrated here, in human cancer patients.

Two-dimensional nanosecond electric field mapping based on cell electropermeabilization

TLDR
This live-cell method for measuring a nanosecond pulsed electric field distribution provides an operationally meaningful calibration of electrode designs for biological applications and permits visualization of the relative sensitivities of different cell types to nanoelectropulse stimulation.
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