A novel immunocytochemical staining method that preserves cell membranes: Application for demonstrating Ca2+ pump ATPase in the liver

@article{Ogi1998ANI,
  title={A novel immunocytochemical staining method that preserves cell membranes: Application for demonstrating Ca2+ pump ATPase in the liver},
  author={Mariko Ogi and Hiroaki Yokomori and Masaya Oda and Masahisa Nakamura and Hiromasa Ashitani Noriaki Ishii and Yoshitaka Kamegaya and Tadashi Motoori},
  journal={Medical Electron Microscopy},
  year={1998},
  volume={31},
  pages={100-106}
}
We have devised a method for immunogold staining of unosmicated, plastic-embedded cells that gives high levels of specific staining without sacrificing cell ultrastucture. Important conditions include PLP (periodate-lysine-paraformaldehyde) fixation, postfixation with uranyl acetate to preserve membrane phospholipids, dehydration with acetone, low-temperature embedding in LR gold resin, and use of osmium tetroxide to stain thin sections after immunogold labeling. We developed this method to… CONTINUE READING

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