A novel hyperglycaemic clamp for characterization of islet function in humans: assessment of three different secretagogues, maximal insulin response and reproducibility.

@article{Fritsche2000ANH,
  title={A novel hyperglycaemic clamp for characterization of islet function in humans: assessment of three different secretagogues, maximal insulin response and reproducibility.},
  author={Andreas Fritsche and Norbert Stefan and Eric Hardt and S Sch{\"u}tzenauer and H. U. H{\"a}ring and Michael Stumvoll},
  journal={European journal of clinical investigation},
  year={2000},
  volume={30 5},
  pages={411-8}
}
BACKGROUND Characterization of beta-cell function in humans is essential for identifying genetic defects involved in abnormal insulin secretion and the pathogenesis of type 2 diabetes. MATERIALS AND METHODS We designed a novel test assessing plasma insulin and C-peptide in response to 3 different secretagogues. Seven lean, healthy volunteers twice underwent a 200 min hyperglycaemic clamp (10 mmol L-1) with administration of GLP-1 (1.5 pmol. kg-1. min-1) starting at 120 min and an arginine… CONTINUE READING