A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae

  title={A novel gene from a secondary metabolism gene cluster is required for microsclerotia formation and virulence in Verticillium dahliae},
  author={Dianguang Xiong and Yonglin Wang and Chengming Tian},
  journal={Phytopathology Research},
Verticillum dahliae is a notorious plant pathogenic fungus, which can cause wilt disease on hundreds of plant species. The genome of V. dahliae has been sequenced and annotated, but incorrect gene annotations and unidentified transcribed regions persist. Using RNA-Seq combined with reverse-transcription PCR, we identified a novel transcribed gene, Nag1, located in a cluster of putative secondary metabolic genes whose roles remain unknown. Functional analysis of Nag1 by dsRNA-mediated gene… 
Opportunities and Challenges in Studies of Host-Pathogen Interactions and Management of Verticillium dahliae in Tomatoes
The current knowledge on the race and population structure of V. dahliae is discussed, including pathogenicity factors, host genes, proteins, enzymes involved in defense, and the emergent management strategies and future research directions for managing Verticillium wilt in tomatoes are discussed.
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This review discusses and summarizes the recent findings on the race and population structure of V. dahliae; pathogenicity factors; host genes, proteins, and enzymes involved in defense; the emergent management strategies, and recent approaches to managing Verticillium wilt in tomatoes.


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Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae
Next generation sequencing technology of RNA-Seq was employed to investigate the global transcriptomic dynamics of MS development to identify differential gene expression at several stages of MS formation in strain XS11 of V. dahliae, and revealed the dynamics of transcriptional regulation during MS formation.
Silencing of Vlaro2 for chorismate synthase revealed that the phytopathogen Verticillium longisporum induces the cross-pathway control in the xylem
The first leaky auxotrophic mutant for aromatic amino acids of the near-diploid fungal plant pathogen Verticillium longisporum has been generated after a novel RNA-mediated gene silencing method reduced gene expression of both isogenes and resulted in a bradytrophic mutant.
Comparative Genomics Yields Insights into Niche Adaptation of Plant Vascular Wilt Pathogens
Insight is revealed into the genetic mechanisms of niche adaptation of fungal wilt pathogens, advances the understanding of the evolution and development of their pathogenesis, and sheds light on potential avenues for the development of novel disease management strategies to combat destructive wilt diseases.
An Ustilago maydis Gene Involved in H2O2 Detoxification Is Required for Virulence[W]
Results suggest that U. maydis is using its Yap1-controlled H2O2 detoxification system for coping with early plant defense responses and functions as a redox sensor.
The APSES transcription factor Vst1 is a key regulator of development in microsclerotium- and resting mycelium-producing Verticillium species.
The functionally characterized the APSES family transcription factor Vst1 in two Verticillium species, V. dahliae and V. nonalfalfae, and found that the absence of vst1 had a great impact on sporulation in both species, affecting the generation of the characteristic verticillate conidiophore structure and sporulation rates in liquid medium.
Functional analysis of non-ribosomal peptide synthetases (NRPSs) in Trichoderma virens reveals a polyketide synthase (PKS)/NRPS hybrid enzyme involved in the induced systemic resistance response in maize.
Evidence is provided that a PKS/NRPS hybrid enzyme is involved in Trichoderma-plant interactions resulting in induction of defence responses, suggesting a putative role for the associated metabolite product in induced systemic resistance.
Verticillium transcription activator of adhesion Vta2 suppresses microsclerotia formation and is required for systemic infection of plant roots.
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