A novel ganglioside with a free amino group in bovine brain.

@article{Hidari1993ANG,
  title={A novel ganglioside with a free amino group in bovine brain.},
  author={Kazuya I.-P. Jwa Hidari and Fumitoshi Irie and Masanori Suzuki and Kazuo Kon and Susumu Ando and Yoshio Hirabayashi},
  journal={The Biochemical journal},
  year={1993},
  volume={296 ( Pt 1)},
  pages={259-63}
}
A novel ganglioside which binds cholera-toxin B-subunit was purified from bovine brain by an h.p.l.c. system using an Aquasil column subsequent to Q-Sepharose column chromatography. T.l.c./immunostaining showed that the isolated ganglioside had about 60% of the binding reactivity of the authentic ganglioside GM1 for cholera-toxin B-subunit. On h.p.l.c., this ganglioside migrated between ganglioside GD1a and GD1b, and was found to give positive reactions with ninhydrin and fluorescamine reagents… CONTINUE READING

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