A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis.

Abstract

Enzyme-linked immunosorbent assay (ELISA) for diagnosis of bovine tuberculosis has been widely explored over the years. Three Mycobacterium bovis-specific antigen genes, namely, mpb70, mpb83, and esat-6 were recombined in tandem by spliced overlap extension technology and expressed in Escherichia coli to obtain the fusion protein (rM70-83-E6). Western blot… (More)

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@article{Liu2007ANF, title={A novel fusion protein-based indirect enzyme-linked immunosorbent assay for the detection of bovine tuberculosis.}, author={Si-guo Liu and Sheping Guo and Chunlai Wang and Meili Shao and Xiuhua Zhang and Yang Guo and Qiang Gong}, journal={Tuberculosis}, year={2007}, volume={87 3}, pages={212-7} }