A novel function of the cAMP-CRP complex in Escherichia coli: cAMP-CRP functions as an adaptor for the CytR repressor in the deo operon.

@article{SgaardAndersen1991ANF,
  title={A novel function of the cAMP-CRP complex in Escherichia coli: cAMP-CRP functions as an adaptor for the CytR repressor in the deo operon.},
  author={Lotte S\ogaard-Andersen and Henrik Holbaek Pedersen and Bj\orn Holst and P. Valentin-Hansen},
  journal={Molecular microbiology},
  year={1991},
  volume={5 4},
  pages={969-75}
}
Unlike classical bacterial repressors, the CytR repressor of Escherichia coli cannot independently regulate gene expression. Here we show that CytR binding to the deoP2 promoter relies on interaction with the master gene regulatory protein, CRP, and, furthermore, that cAMP-CRP and CytR bind co-operatively to deoP2. Using mutant promoters we show that tandem, properly spaced DNA-bound cAMP-CRP complexes are required for this co-operative binding. These data suggest that CytR forms a bridge… CONTINUE READING
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