A novel follicle culture system markedly increases follicle volume, cell number and oestradiol secretion.

@article{Wycherley2004ANF,
  title={A novel follicle culture system markedly increases follicle volume, cell number and oestradiol secretion.},
  author={G Wycherley and D{\'o}nal Downey and Michael T. Kane and Ailish C Hynes},
  journal={Reproduction},
  year={2004},
  volume={127 6},
  pages={669-77}
}
This study reports a novel, simple method for culture of mouse follicles which results in follicles with cell numbers similar to in vivo fully grown follicles. Using this method, follicles (180-240 microm in diameter) were cultured in a 100 microl inverted drop of medium without oil and compared with culture in upright drops with and without a mineral oil overlay. Follicles, isolated from C57BL/6 x CBA/ca crossbred and MF1 inbred mice, were cultured individually at 37 degrees C in 96-well round… CONTINUE READING