A novel fluorimetric method for laccase activities measurement using Amplex Red as substrate.

Abstract

In this paper, a novel fluorescence-based method for laccase assay was presented. The method was based on the transformation of Amplex Red into a highly fluorescent and colored resorufin catalyzed by laccase in the presence of O2. The catalysis and transformation mechanism were investigated in detail. The kinetic parameters of the Amplex Red catalysis by… (More)
DOI: 10.1016/j.talanta.2016.10.006

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