A novel fluorescence assay for the detection of hemoglobin based on the G-quadruplex/hemin complex.

@article{Pang2014ANF,
  title={A novel fluorescence assay for the detection of hemoglobin based on the G-quadruplex/hemin complex.},
  author={Shu Pang and Siyu Liu and Xingguang Su},
  journal={Talanta},
  year={2014},
  volume={118},
  pages={118-22}
}
A novel sensing strategy for specific recognition of hemoglobin has been designed. In the presence of trypsin, hemoglobin was cleaved to release heme which could effectively bind to a FITC-labeled guanine (G)-rich oligonucleotide sequence to form the G-quadruplex/hemin complex. This binding process would lead to fluorescence quenching of FITC via photoinduced electron transfer. Therefore, a fluorescence assay for the determination of hemoglobin could be established. Under the optimized… CONTINUE READING

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HemeNo subtypeHemin
In the presence of trypsin , hemoglobin was cleaved to release heme which could effectively bind to a FITC - labeled guanine ( G)-rich oligonucleotide sequence to form the G - quadruplex / hemin complex .
HeminNo subtypeHeme
In the presence of trypsin , hemoglobin was cleaved to release heme which could effectively bind to a FITC - labeled guanine ( G)-rich oligonucleotide sequence to form the G - quadruplex / hemin complex .
HeminNo subtypeHeme
In the presence of trypsin , hemoglobin was cleaved to release heme which could effectively bind to a FITC - labeled guanine ( G)-rich oligonucleotide sequence to form the G - quadruplex / hemin complex .
In the presence of trypsin , hemoglobin was cleaved to release heme which could effectively bind to a FITC - labeled guanine ( G)-rich oligonucleotide sequence to form the G - quadruplex / hemin complex .
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