A novel flow cytometric method for quantifying phagocytosis of apoptotic cells.

@article{Hess1997ANF,
  title={A novel flow cytometric method for quantifying phagocytosis of apoptotic cells.},
  author={Krista L Hess and George F. Babcock and Diana Askew and Joan M. Cook-Mills},
  journal={Cytometry},
  year={1997},
  volume={27 2},
  pages={145-52}
}
Many eukaryotic cell types are capable of specific recognition and phagocytosis of apoptotic cells, and there is increasing interest in the mechanisms involved in this process. To facilitate analysis of these mechanisms, we designed a novel fluorescence-based method to quantify phagocytosis in vitro using endothelial cell engulfment of apoptotic cells as a model. The B-cell line WEHI-231 was labeled with the fluorophore 5-(&-6)-carboxytetramethyl-rhodamine-succinimidyl-ester (TAMRA) and then… CONTINUE READING

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