A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet

@article{Sumi2005ANF,
  title={A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet},
  author={Hiroyuki Sumi and Hiroki Hamada and Hirofumi Tsushima and Hisashi Mihara and H. Muraki},
  journal={Experientia},
  year={2005},
  volume={43},
  pages={1110-1111}
}
A strong fibrinolytic activity was demonstrated in the vegetable cheese Natto, which is a typical soybean food eaten in Japan. The average activity was calculated at about 40 CU (plasmin units)/g wet weight. This novel fibrinolytic enzyme, named nattokinase, was easily extracted with saline. The mol. wt and pI were about 20,000 and 8.6, respectively. Nattokinase not only digested fibrin but also the plasmin substrate H-D-Val-Leu-Lys-pNA (S-2251), which was more sensitive to the enzyme than… 
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References

SHOWING 1-8 OF 8 REFERENCES
Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins.
TLDR
Oral administration of clinical-type high molecular weight urokinase in a single dose of 30,000-60,000 International Units (IU) in enteric-coated capsules induced a plasma fibrinolytic state suggesting transport of HMW-UK across the intestinal tract, shown to be clinically effective in patients with cerebral thrombosis in a multicenter double blind study.
Methods for determination of prekallikrein in plasma, glandular kallikrein and urokinase.
TLDR
New chromogenic tripeptide substrates have been used for the determination of kallikreins and urokinase and it is possible to determine prek allikrein in plasma after activation with Cephotest.
The fibrin plate method for estimating fibrinolytic activity.
Oral administration of urokinase.