A novel esterase from Saccharomyces carlsbergensis, a possible function for the yeast TIP1 gene.

@article{Horsted1998ANE,
  title={A novel esterase from Saccharomyces carlsbergensis, a possible function for the yeast TIP1 gene.},
  author={M W Horsted and E S Dey and Steen Holmberg and Morten C. Kielland-Brandt},
  journal={Yeast},
  year={1998},
  volume={14 9},
  pages={793-803}
}
An extracellular esterase was isolated from the brewer's yeast, Saccharomyces carlsbergensis. Inhibition by diisopropyl fluorophosphate shows that the enzyme has a serine active site. By mass spectrometry, the molecular weight of the enzyme was 16.9 kDa. The optimal pH for activity was in the range of four to five. Esterase activity was found in beer before pasteurization, and a low level of activity was still present after pasteurization. Caprylic acid, which is present in beer, competitively… CONTINUE READING