A novel enzyme, citryl‐CoA lyase, catalysing the second step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK‐6

@article{Aoshima2004ANE,
  title={A novel enzyme, citryl‐CoA lyase, catalysing the second step of the citrate cleavage reaction in Hydrogenobacter thermophilus TK‐6},
  author={M. Aoshima and M. Ishii and Y. Igarashi},
  journal={Molecular Microbiology},
  year={2004},
  volume={52}
}
  • M. Aoshima, M. Ishii, Y. Igarashi
  • Published 2004
  • Biology, Medicine
  • Molecular Microbiology
  • A novel enzyme catalysing citryl‐CoA cleavage to acetyl‐CoA and oxaloacetate was purified from Hydrogenobacter thermophilus TK‐6, and designated citryl‐CoA lyase (CCL). The citrate cleavage reaction in this organism proceeded by a unique set of two consecutive reactions: (i) citryl‐CoA formation by citryl‐CoA synthetase (CCS) and (ii) citryl‐CoA cleavage by CCL. Purified CCL gave a single 30 kDa band in SDS–PAGE and gel filtration chromatography indicated that the native state of the enzyme… CONTINUE READING
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