A novel endonuclease mechanism directly visualized for I-PpoI

@article{Galburt1999ANE,
  title={A novel endonuclease mechanism directly visualized for I-PpoI},
  author={Eric A Galburt and Brett Smith Chevalier and Weiliang Tang and Melissa S. Jurica and Karen E. Flick and Raymond J. Monnat and Barry L. Stoddard},
  journal={Nature Structural Biology},
  year={1999},
  volume={6},
  pages={1096-1099}
}
A novel mechanism of DNA endonucleolytic cleavage has been visualized for the homing endonuclease I-PpoI by trapping the uncleaved enzyme–substrate complex and comparing it to the previously visualized product complex. This enzyme employs a unique single metal mechanism. A magnesium ion is coordinated by an asparagine residue and two DNA oxygen atoms and stabilizes the phosphoanion transition state and the 3'oxygen leaving group. A hydrolytic water molecule is activated by a histidine residue… CONTINUE READING
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