A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.

@article{Ghadessy2004ANE,
  title={A novel emulsion mixture for in vitro compartmentalization of transcription and translation in the rabbit reticulocyte system.},
  author={Farid J Ghadessy and Philipp Holliger},
  journal={Protein engineering, design & selection : PEDS},
  year={2004},
  volume={17 3},
  pages={201-4}
}
Emulsion formulations used for in vitro compartmentalization (IVC) methods were found to be incompatible with protein expression in the rabbit reticulocyte (RRL) system, causing rapid discoloration and translation shutdown. Here we identify possible causes and describe a novel water-in-oil emulsion which abolished discoloration and allowed high-level in… CONTINUE READING