A novel degradative pathway of 2-nitrobenzoate via 3-hydroxyanthranilate in Pseudomonas fluorescens strain KU-7.

@article{Hasegawa2000AND,
  title={A novel degradative pathway of 2-nitrobenzoate via 3-hydroxyanthranilate in Pseudomonas fluorescens strain KU-7.},
  author={Yasuchika Hasegawa and Takamichi Muraki and Tatsuaki Tokuyama and Hiroaki Iwaki and Michiaki Tatsuno and Peter C. K. Lau},
  journal={FEMS microbiology letters},
  year={2000},
  volume={190 2},
  pages={
          185-90
        }
}
A bacterial strain KU-7, identified as a Pseudomonas fluorescens by 16S rDNA sequencing, was one of the 12 new isolates that are able to grow on 2-nitrobenzoate as a sole source of carbon, nitrogen, and energy. Resting cells of KU-7 were found to accumulate ammonia in the medium indicating that degradation of 2-NBA proceeds through a reductive route. Metabolite analyses by thin layer chromatography and high pressure liquid chromatography indicated that 3-hydroxyanthranilate is an intermediate… CONTINUE READING
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