A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development

Abstract

To establish an in vitro culture system for mouse round spermatids that models spermiogenesis and enables the assessment of oocyte activation ability. Round spermatids and Sertoli cells were isolated from testicular tissues of B6D2F1 male mice and co-cultured in the presence of testosterone and recombinant FSH. Cultured spermatids were examined for… (More)
DOI: 10.1007/s10815-010-9442-3

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@article{Hasegawa2010ANC, title={A novel culture system for mouse spermatid maturation which produces elongating spermatids capable of inducing calcium oscillation during fertilization and embryonic development}, author={Hisataka Hasegawa and Yukihiro Terada and Tomohisa Ugajin and Nobuo Yaegashi and Kahei Sato}, journal={Journal of Assisted Reproduction and Genetics}, year={2010}, volume={27}, pages={565-570} }