A novel cry2Ab gene from the indigenous isolate Bacillus thuringiensis subsp. kurstaki.

@article{Sevim2012ANC,
  title={A novel cry2Ab gene from the indigenous isolate Bacillus thuringiensis subsp. kurstaki.},
  author={Ali Sevim and Emine Ery{\"u}zl{\"u} and Zihni Demirbağ and Ismail Demir},
  journal={Journal of microbiology and biotechnology},
  year={2012},
  volume={22 1},
  pages={
          133-40
        }
}
A novel cry2Ab gene was cloned and sequenced from the indigenous isolate of Bacillus thuringiensis subsp. kurstaki. This gene was designated as cry2Ab25 and its sequence revealed an open reading frame of 1,902 bp encoding a 633 aa protein with calculated molecular mass of 70 kDa and pI value of 8.98. The amino acid sequence of the Cry2Ab25 protein was compared with previously known Cry2Ab toxins, and the phylogenetic relationships among them were determined. The deduced amino acid sequence of… 
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