A novel colorimetric assay and activity staining for cytokinin oxidase based on the copper amine oxidase property of the enzyme.

Abstract

A rapid and sensitive colorimetric assay for cytokinin oxidase (CKO) was developed based on the copper amine oxidase property of the enzyme from cucumber cotyledons. The assay involved oxidation of isopentenyl adenine by CKO in the presence of phenazine methosulphate as an electron acceptor, resulting in reduction of nitroblue tetrazolium to formazon. The… (More)

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@article{Kulkarni2001ANC, title={A novel colorimetric assay and activity staining for cytokinin oxidase based on the copper amine oxidase property of the enzyme.}, author={Martand Kulkarni and D Theerthaprasad and L. Sudharshana and Trichi Ganesh Prasad and V. R. Sashidhar}, journal={Phytochemical analysis : PCA}, year={2001}, volume={12 3}, pages={180-4} }