A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

  title={A novel cold active esterase derived from Colombian high Andean forest soil metagenome.},
  author={Diego Javier Jim{\'e}nez and Jos{\'e} Salvador Monta{\~n}a and Diana {\'A}lvarez and Sandra Baena},
  journal={World journal of microbiology & biotechnology},
  volume={28 1},
In order to search new lipolytic enzymes and conduct bioprospecting of microbial communities from high Andean forest soil, a metagenomic library of approximately 20,000 clones was constructed in Escherichia coli using plasmid p-Bluescript II SK+. The library covered 80 Mb of the metagenomic DNA mainly from Proteobacteria, Actinobacteria and Acidobacteria. Two clones with lipolytic activity in tributyrin as a substrate were recovered. Clone BAA3G2 (pSK-estGX1) was selected and the entire 4.6 Kb… CONTINUE READING
Highly Cited
This paper has 212 citations. REVIEW CITATIONS
10 Citations
63 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 10 extracted citations

213 Citations

Citations per Year
Semantic Scholar estimates that this publication has 213 citations based on the available data.

See our FAQ for additional information.


Publications referenced by this paper.
Showing 1-10 of 63 references

Global market for industrial enzymes to reach

  • M Rajan
  • 2004
Highly Influential
2 Excerpts

White biotechnology: gateway to a more sustainable future [online] http://www.mckinsey.com/ clientservice/chemicals/pdf/BioVision_Booklet_final.pdf

  • H Schepens
  • 2003
Highly Influential
3 Excerpts

Similar Papers

Loading similar papers…