A novel cold active esterase derived from Colombian high Andean forest soil metagenome.

@article{Jimnez2012ANC,
  title={A novel cold active esterase derived from Colombian high Andean forest soil metagenome.},
  author={Diego Javier Jim{\'e}nez and Jos{\'e} Salvador Monta{\~n}a and Diana {\'A}lvarez and Sandra Baena},
  journal={World journal of microbiology & biotechnology},
  year={2012},
  volume={28 1},
  pages={361-70}
}
In order to search new lipolytic enzymes and conduct bioprospecting of microbial communities from high Andean forest soil, a metagenomic library of approximately 20,000 clones was constructed in Escherichia coli using plasmid p-Bluescript II SK+. The library covered 80 Mb of the metagenomic DNA mainly from Proteobacteria, Actinobacteria and Acidobacteria. Two clones with lipolytic activity in tributyrin as a substrate were recovered. Clone BAA3G2 (pSK-estGX1) was selected and the entire 4.6 Kb… CONTINUE READING
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Global market for industrial enzymes to reach

  • M Rajan
  • 2004
Highly Influential
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White biotechnology: gateway to a more sustainable future [online] http://www.mckinsey.com/ clientservice/chemicals/pdf/BioVision_Booklet_final.pdf

  • H Schepens
  • 2003
Highly Influential
3 Excerpts

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