A novel cleavage site within the potato leafroll virus P1 polyprotein.

@article{Li2007ANC,
  title={A novel cleavage site within the potato leafroll virus P1 polyprotein.},
  author={Xuejun Li and Claire Halpin and Martin D Ryan},
  journal={The Journal of general virology},
  year={2007},
  volume={88 Pt 5},
  pages={1620-3}
}
To study the proteolytic processing of the potato leafroll virus replicase proteins, the multidomain P1 protein with a c-myc epitope tag attached at the N terminus was expressed in insect cells by using the baculovirus system. Western blotting showed that P1 was cleaved at a site upstream of the serine protease domain, in addition to the cleavage site downstream of the protease domain. Mutational analysis showed that the serine protease domain within P1 was responsible for this cleavage. To… CONTINUE READING