A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity.

@article{Lee2005ANC,
  title={A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity.},
  author={Seong Jin Lee and Kwang Sik Lee and Seong-Ryul Kim and Zhongzheng Gui and Yong Soon Kim and Hyung Joo Yoon and Iksoo Kim and Pil-Don Kang and Hung Dae Sohn and Byung Rae Jin},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry \& molecular biology},
  year={2005},
  volume={140 4},
  pages={
          551-60
        }
}
  • S. LeeK. Lee B. Jin
  • Published 1 April 2005
  • Biology
  • Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
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67 Citations
Highly Influential Citations
4
Background Citations
25
Methods Citations
5
Results Citations
1

67 Citations

Molecular cloning, expression, and enzymatic activity of a novel endogenous cellulase from the mulberry longicorn beetle, Apriona germari.

  • Y. WeiK. Lee B. Jin
  • Biology
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2006

cDNA cloning, expression, and enzymatic activity of a novel endogenous cellulase from the beetle Batocera horsfieldi.

Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean

The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and successful cloning of EG27 cDNA supported endogenous production of EG 27 cellulase by Ampullaria crossean.

N-linked glycosylation of a beetle (Apriona germari) cellulase Ag-EGase II is necessary for enzymatic activity.

  • Y. WeiK. Lee B. Jin
  • Biology, Chemistry
    Insect biochemistry and molecular biology
  • 2006

Molecular cloning, expression, purification and characterization of a novel cellulase gene (Bh-EGaseI) in the beetle Batocera horsfieldi.

Molecular cloning and characterization of a glycosyl hydrolase family 9 cellulase distributed throughout the digestive tract of the cricket Teleogryllus emma.

  • Namjung KimY. Choo B. Jin
  • Biology
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2008

Identification, cloning, and expression of a GHF9 cellulase from Tribolium castaneum (Coleoptera: Tenebrionidae).

Purification and Characterization of Recombinant Endoglucanases from the Pine Wood Nematode Bursaphelenchus xylophilus

The purification and characterization of the recombinant enzymes, named Bx-ENG-1, 2, and 3, expressed in Pichia pastoris suggest that B. xylophilus endoglucanases acted on the cellulose-hemicellulose complex in the cell walls, resulting in a weakening of the mechanical strength of the cell Walls to facilitate the nematode’s feeding on plant cells.

Endogenous cellulase production in the leaf litter foraging mangrove crab Parasesarma erythodactyla.

cDNA cloning, homology modelling and evolutionary insights into novel endogenous cellulases of the borer beetle Oncideres albomarginata chamela (Cerambycidae)

Phylogenetic analyses suggest that insect GHF5 and GHF45 are very ancient gene families and indicate, at least in the case ofGHF5, that this family likely evolved from a common ancestor rather than, as is often reported, via horizontal gene transfer.
...

References

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cDNA cloning, expression, and enzymatic activity of a cellulase from the mulberry longicorn beetle, Apriona germari.

  • S. LeeSeong-Ryul Kim B. Jin
  • Biology
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
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Metazoan cellulase genes from termites: intron/exon structures and sites of expression.

Homology between cellulase genes of Trichoderma reesei: complete nucleotide sequence of the endoglucanase I gene.

Purification, characterization, cDNA cloning and nucleotide sequencing of a cellulase from the yellow-spotted longicorn beetle, Psacothea hilaris.

TLC analysis showed that the cellulase produces cellotriose and cellobiose from insoluble cello-oligosaccharides, which is the first report of a family 5 cellulase from arthropods.

Cloning, Characterization, and Expression in Saccharomyces Cerevisiae of Endoglucanase I from Trichoderma Reesei

DNA sequencing revealed significant homology at the amino acid level between EGI and exocellobiohydrolase I, but there are differences in codon utilization at homologous amino acids and in the intron/exon structure that possibly reflect a mechanism for preventing recombination between closely related genes of the cellulase family.

Diverse genes of cellulase homologues of glycosyl hydrolase family 45 from the symbiotic protists in the hindgut of the termite Reticulitermes speratus

The presence of diverse cellulase homologues suggests that symbiotic protists of termites may be rich reservoirs of novel cellulase sequences.

Molecular cloning of three cDNAs encoding putative larval cuticle protein expressed differentially after larval ecdysis from the mulberry longicorn beetle, Apriona germari.

  • Seong-Ryul KimH. Yoon B. Jin
  • Biology, Medicine
    Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
  • 2003

Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae.

Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis.

The specific activities of the intact enzymes and their cores on two forms of insoluble cellulose (crystalline, amorphous) differentiate the CBH I and CBH II in terms of adsorption and catalytic properties.

Molecular Cloning of a cDNA Encoding a Cathepsin B Homologue from the Mulberry Longicorn Beetle, Apriona germari

Northern blot analysis suggests that A. germari cathepsin D homologue gene is expressed in most if not all, body tissues, and appears to have two putative glycosylation sites, rather than one.