A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity.

@article{Lee2005ANC,
  title={A novel cellulase gene from the mulberry longicorn beetle, Apriona germari: gene structure, expression, and enzymatic activity.},
  author={Seong Jin Lee and Kwang Sik Lee and S. R. Kim and Zhongzheng Gui and Yong Soon Kim and Hyung Joo Yoon and Iksoo Kim and Pil Don Kang and Hung Dae Sohn and Byung Rae Jin},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={2005},
  volume={140 4},
  pages={551-60}
}
We have previously cloned a cellulase [beta-1,4-endoglucanase (EGase), EC 3.2.1.4] cDNA (Ag-EGase I) belonging to glycoside hydrolase family (GHF) 45 from the mulberry longicorn beetle, Apriona germari. We report here the gene structure, expression and enzyme activity of an additional celluase (Ag-EGase II) from A. germari and also described the gene structure of Ag-EGase I. The Ag-EGase II gene spans 1033 bp and consisted of two introns and three exons coding for 239 amino acid residues. The… CONTINUE READING

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