A novel cationic cholesterol derivative, its formulation into liposomes, and the efficient transfection of the transformed human cell lines HepG2 and HeLa.

@article{Kisoon2002ANC,
  title={A novel cationic cholesterol derivative, its formulation into liposomes, and the efficient transfection of the transformed human cell lines HepG2 and HeLa.},
  author={Neermala Kisoon and Mario Ariatti and Thesegen Moodley},
  journal={Drug delivery},
  year={2002},
  volume={9 3},
  pages={
          161-7
        }
}
A novel cationic cholesterol derivative, 3beta[N-(N',N',N'-trimethylaminopropane)-carbamoyl] cholesterol iodide (Chol-Q), has been formulated with equimolar amounts of dioleoyl phosphatidylethanolamine (DOPE) into stable unilamellar liposomes up to 100 nm in size for DNA delivery into mammalian cells. When compared with similarly constituted liposomes containing the tertiary analogue 3beta[N-(N',N'-dimethylaminopropane)-carbamoyl] cholesterol (Chol-T) in a band shift assay, liposomes displayed… CONTINUE READING
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