A novel carotenoid, 4-keto-α-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus.

@article{Breitenbach2014ANC,
  title={A novel carotenoid, 4-keto-α-carotene, as an unexpected by-product during genetic engineering of carotenogenesis in rice callus.},
  author={J{\"u}rgen Breitenbach and Chao Bai and Sol Maiam Rivera and Ramon Canela and Teresa Capell and Paul Christou and Changfu Zhu and G erhard Sandmann},
  journal={Phytochemistry},
  year={2014},
  volume={98},
  pages={85-91}
}
Rice endosperm is devoid of carotenoids because the initial biosynthetic steps are absent. The early carotenogenesis reactions were constituted through co-transformation of endosperm-derived rice callus with phytoene synthase and phytoene desaturase transgenes. Subsequent steps in the pathway such as cyclization and hydroxylation reactions were catalyzed by endogenous rice enzymes in the endosperm. The carotenoid pathway was extended further by including a bacterial ketolase gene able to form… CONTINUE READING