A novel carbonic anhydrase II binding site regulates NHE1 activity.

@article{Li2006ANC,
  title={A novel carbonic anhydrase II binding site regulates NHE1 activity.},
  author={Xiuju Li and Yong-sheng Liu and Bernardo V. Alvarez and Joseph R Casey and Larry Fliegel},
  journal={Biochemistry},
  year={2006},
  volume={45 7},
  pages={2414-24}
}
Carbonic anhydrase II (CAII) binds to and regulates transport by the NHE1 isoform of the mammalian Na(+)/H(+) exchanger. We localized and characterized the CAII binding region on the C-terminal tail of the Na(+)/H(+) exchanger. CAII did not bind to acidic sequences in NHE1 that were similar to the CAII binding site of bicarbonate transporters. Instead, by expressing a variety of fusion proteins of the C-terminal region of the Na(+)/H(+) exchanger, we demonstrated that CAII binds to the… CONTINUE READING