A novel autoantibody causing a peripheral fluorescent antinuclear antibody pattern is specific for nuclear pore complexes.

@article{Dagenais1988ANA,
  title={A novel autoantibody causing a peripheral fluorescent antinuclear antibody pattern is specific for nuclear pore complexes.},
  author={Andr{\'e} Dagenais and V Bibor-Hardy and J. L. Senecal},
  journal={Arthritis and rheumatism},
  year={1988},
  volume={31 10},
  pages={1322-7}
}
We characterized serum from a patient with polymyositis, and found that it produced a peripheral (rim) fluorescent antinuclear antibody pattern on rat liver substrate. Indirect immunofluorescence analysis revealed a punctate pattern at the nuclear surface of PtK2, BHK-21, and HEp-2 cells. This pattern was still present after sequential extraction in situ with non-ionic detergent, DNase, RNase, and high ionic strength buffer (2M NaCl). Immunogold electron microscopic localization was specific… CONTINUE READING