A novel assay for the measurement of Raf-1 kinase activity

  title={A novel assay for the measurement of Raf-1 kinase activity},
  author={Cornelius Bondzi and Steven Grant and Geoffrey W. Krystal},
Raf-1 is a serine-threonine protein kinase that functions as a central component of the mitogen-activated protein kinase signal transduction pathway. Raf-1 activity is currently assayed in vitro by either measuring 32P incorporation into MEK, Raf-1’s only characterized substrate, or by using the phosphorylated MEK to initiate a coupled assay culminating in the phosphorylation of myelin basic protein by MAP kinase. These assays are plagued by a potential lack of specificity in the case of the… CONTINUE READING
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