A novel approach to monitor stress-induced physiological responses in immobilized microorganisms

@article{Alonso2015ANA,
  title={A novel approach to monitor stress-induced physiological responses in immobilized microorganisms},
  author={Sa{\'u}l Alonso and Manuel Rendueles and Mario D{\'i}az},
  journal={Applied Microbiology and Biotechnology},
  year={2015},
  volume={99},
  pages={3573-3583}
}
Microbial cell immobilization has long been considered as a potential bioprocessing strategy to increase both microorganisms’ tolerance and fitness in fermentation systems. To date, little emphasis has been put on how the entrapped cells respond to the bioprocessing stresses encountered during the cultivation. The present work presents for the first time a methodology to decipher the real health status of the entrapped microorganisms by combining multiparameter flow cytometry with confocal… 

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