A novel approach to monitor stress-induced physiological responses in immobilized microorganisms

  title={A novel approach to monitor stress-induced physiological responses in immobilized microorganisms},
  author={Sa{\'u}l Alonso and Manuel Rendueles and Mario D{\'i}az},
  journal={Applied Microbiology and Biotechnology},
Microbial cell immobilization has long been considered as a potential bioprocessing strategy to increase both microorganisms’ tolerance and fitness in fermentation systems. To date, little emphasis has been put on how the entrapped cells respond to the bioprocessing stresses encountered during the cultivation. The present work presents for the first time a methodology to decipher the real health status of the entrapped microorganisms by combining multiparameter flow cytometry with confocal… 

Microencapsulated Starter Culture During Yoghurt Manufacturing, Effect on Technological Features

Study of the effects of chitosan-alginate capsules on metabolic activities of Streptococcus thermophilus and probiotic Lactobacillus delbrueckii during milk fermentation for yoghurt production indicates that both kinds of capsules influenced at different extent cell functionalities (growth, acidification and proteolysis), while they improve cell viability during yoghur storage and simulated gastrointestinal passage.

Biohydrogen Production by Reusing Immobilized Mixed Culture in Batch System

The results showed that the colonies growth observed using optical microscopy or SEM was apparent only in the immobilization of mixed culture, and the reuse of alginate beads affecting the biohydrogen production for 45 days was evaluated.

Exploring encapsulation strategies as a protective mechanism to avoid amensalism in mixed populations of Pseudomonas taetrolens and Lactobacillus casei

The potential of LAB encapsulation for the protection of sensitive strains in mixed food fermentations is proved, improving the production of lactobionic acid by 182% with respect to co-cultures with free cells.

Novel Preservation Techniques for Microbial Cultures

The recent advances in the preservation of microbial cultures are addressed, highlighting the relevance of emerging methods on maintaining the cellular viability and how novel preservation methods can shield the microbial cells from undesired stress responses.

In Situ and Contact Immobilization/Adhesion of Penicillium brasilianum in Polyurethane Foam for Production of Exo-Polygalacturonase

The present work aimed to immobilize Penicillium brasilianum fungus in situ or by contact in polyurethane foam and assess the production of exo-polygalacturonase with the immobilized fungus while e...

Novel Food Fermentation Technologies

This book reiterates that the fermented products are relevant to modern society and has evolved over centuries. This book has demonstrated that the application of novel microbial cultures and novel

Synthesis of Xanthan Gum fromXanthomonas campestrisImmobilized in Polyurethane

Abstract Xanthan gum, a biopolymer produced by the bacteria Xanthomonas campestris, with varied applications in many industrial branches. Cell immobilization is an alternative to enhance xanthan gu...

High-sodium maltobionate production by immobilized Zymomonas mobilis cells in polyurethane

The immobilized system showed stability for 19 successive operational cycles of 24 h bioconversion and 6 months of storage, at 4 °C or 22 °C, for the production of maltobionic acid by Z. mobilis cells immobilized in polyurethane.



Physiological heterogeneity of Pseudomonas taetrolens during lactobionic acid production

This study provides the most suitable criteria for optimizing lactobionic acid production efficiency through a novel flow cytometric-based approach based on the physiological status of P. taetrolens and constitutes a valuable, broad-ranging methodology for the enhancement of microbial bioprocesses involved in the production of secondary metabolites.

Biosurfactant production by free and alginate entrapped cells of Pseudomonas fluorescens

The biosurfactant showed a rhamnolipid-type in nature, and had a good foaming and emulsifying activities, and showed good stability during exposure to high temperatures, to high salinity and to a wide range of pH (4–9).

Application of flow cytometry to industrial microbial bioprocesses

Effects of encapsulation of microorganisms on product formation during microbial fermentations

Fermentation, using encapsulated cells, offers various advantages compared to traditional cultivations, e.g., higher cell density, faster fermentation, improved tolerance of the cells to toxic media and high temperatures, and selective exclusion of toxic hydrophobic substances.

Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors

A model is presented, where the stress response is induced by nutrient limitation, that this helps the cells to cope with the increased stress added by a toxic medium, and that superficial cells in the capsules degrade convertible inhibitors, alleviating the inhibition for the cells deeper in the capsule.

The application of multi-parameter flow cytometry to monitor individual microbial cell physiological state.

Using multi-parameter flow cytometric techniques it is possible to resolve a cells physiological state, beyond culturability to include metabolic activity enabling assessment of population heterogeneity, and results are available in real-time, 1-2 min after a sample is taken, enabling informed decisions about a process.

Alginate beads provide a beneficial physical barrier against native microorganisms in wastewater treated with immobilized bacteria and microalgae

It is demonstrated that wastewater microbial populations are responsible for decreasing populations of biological agents used for wastewater treatment and immobilization in alginate beads provided a protective environment for these agents to carry out uninterrupted tertiary wastewater treatment.

A way to follow the viability of encapsulated Bifidobacterium bifidum subjected to a freeze-drying process in order to target the colon: interest of flow cytometry.

  • F. Martin-DejardinB. Ebel O. Chambin
  • Biology, Medicine
    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
  • 2013