A novel approach to monitor intracellular degradation kinetics of poly(lactide-co-glycolide) nanoparticles by means of flow cytometry.

@article{Romero2014ANA,
  title={A novel approach to monitor intracellular degradation kinetics of poly(lactide-co-glycolide) nanoparticles by means of flow cytometry.},
  author={Gabriela Romero and Mar{\'i}a Echeverr{\'i}a and Yuan Qiu and Richard A Murray and Sergio Enrique Moya},
  journal={Journal of materials chemistry. B},
  year={2014},
  volume={2 7},
  pages={
          826-833
        }
}
The intracellular degradation of poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) is studied by means of flow cytometry (FACS). NPs are prepared with PLGA of two different ratios of the d,l-lactide and glycolide blocks: 85 : 15 and 65 : 35. PLGA molecules are labelled with rhodamine B. Flow cytometry is first used to follow the degradation of PLGA NPs in PBS over time by measuring the decrease in fluorescence per particle. The 85 : 15 PLGA NPs progressively degrade during the first 10 days… 
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