A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency

@inproceedings{Tharkeshwar2017ANA,
  title={A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency},
  author={Arun Kumar Tharkeshwar and Jesse Trekker and Wendy Vermeire and Jarne Pauwels and Ragna Sannerud and David A. Priestman and Danielle te Vruchte and Katlijn Vints and Pieter Baatsen and Jean-Paul Decuypere and Huiqi Lu and Shaun Martin and Peter Vangheluwe and Johannes V Swinnen and Liesbet Lagae and Francis Impens and Frances M Platt and Kris Gevaert and Wim Annaert},
  booktitle={Scientific reports},
  year={2017}
}
Superparamagnetic iron oxide nanoparticles (SPIONs) have mainly been used as cellular carriers for genes and therapeutic products, while their use in subcellular organelle isolation remains underexploited. We engineered SPIONs targeting distinct subcellular compartments. Dimercaptosuccinic acid-coated SPIONs are internalized and accumulate in late endosomes/lysosomes, while aminolipid-SPIONs reside at the plasma membrane. These features allowed us to establish standardized magnetic isolation… CONTINUE READING