A novel approach for the improvement of ethanol fermentation by Saccharomyces cerevisiae.

@article{Hou2010ANA,
  title={A novel approach for the improvement of ethanol fermentation by Saccharomyces cerevisiae.},
  author={Li-hua Hou and Xiaohong Cao and Chun-Ling Wang},
  journal={Canadian journal of microbiology},
  year={2010},
  volume={56 6},
  pages={495-500}
}
Fermentation properties under the control of multiple genes are difficult to alter with traditional methods in Saccharomyces cerevisiae. Here, a novel genome engineering approach is developed to improve ethanol production in very high gravity fermentation with 300 g/L glucose as the carbon source. This strategy involved constructing aneuploid strains on the base of tetraploid cells. The tetraploid strain was constructed by using the plasmid YCplac33-GHK, which harbored the HO gene encoding the… CONTINUE READING