A novel approach for expression cloning of small GTPases: identification, tissue distribution and chromosome mapping of the human homolog of rheb

@article{Gromov1995ANA,
  title={A novel approach for expression cloning of small GTPases: identification, tissue distribution and chromosome mapping of the human homolog of rheb},
  author={P. S. Gromov and Peder Madsen and N Tomerup and J. E. Celis},
  journal={FEBS Letters},
  year={1995},
  volume={377}
}
An expression cloning method to identify monomeric GTP-binding proteins by GTP overlay.
TLDR
This work cloned a series of small Ras-like GTP-binding proteins from human retina using a method based on probing plasmid expression libraries with [alpha-32P]GTP based on a functional test, ligand specificity of the expressed proteins, to identify candidate molecules.
Identification and Characterization of RHEBL1, a Novel Member of Ras Family, which Activates Transcriptional Activities of NF-Kappa B
TLDR
The findings suggest that RHEBL1 is a positive regulator of NF-kappa B-mediated gene transcription.
Expression, purification, crystallization and preliminary structural characterization of the GTPase domain of human Rheb.
TLDR
Structural studies of hRheb in complexes with various substrates may provide insights into the recognition and specificity of substrate and the catalytic mechanism of mammalian Rheb and shed light on the biological functions of Rhebs in the mTOR signalling pathway.
Identification of Dominant Negative Mutants of Rheb GTPase and Their Use to Implicate the Involvement of Human Rheb in the Activation of p70S6K*
TLDR
Identification of dominant negative mutants of S. pombe Rheb (SpRheb) provides critical evidence that RheB plays a role in the mTOR/S6K pathway in mammalian cells.
Journal of Biotech Research [ISSN: 1944-3285]
TLDR
This work has developed and characterized a highly specific and potent affinity-purified antibody toward the mouse RhebL1 protein that is not ubiquitously expressed in mouse tissues and that the brain exhibits the highest level of expression.
Structure and Function of the mTOR Activator Rheb
TLDR
The structure and function of Rheb is described, its role in mTOR signaling and noncanonical functions, as well as its physiological importance in health and disease are described.
The Saccharomyces cerevisiae Rheb G-protein Is Involved in Regulating Canavanine Resistance and Arginine Uptake*
TLDR
The regulation of arginine and lysine uptake is assigned as the first physiological function for this new farnesylated Ras superfamily G-protein.
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