A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis.

@article{Gonalves2012ANA,
  title={A novel approach based on antigen, antibody and immune complex detection in bronchoalveolar lavage fluid samples from rats experimentally infected with Strongyloides venezuelensis.},
  author={Ana L{\'u}cia Ribeiro Gonçalves and Thamy S. Ribeiro and Claudio Vieira da Silva and Marlene Ueta and Julia Mar{\'i}a Costa-Cruz},
  journal={Acta tropica},
  year={2012},
  volume={124 2},
  pages={166-9}
}
This study was performed in order to develop a novel approach based on antigen, antibody and immune complex detection by enzyme-linked immunosorbent assay (ELISA) in bronchoalveolar lavage fluid (BALF) samples. For that purpose Wistar rats immunosuppressed or not were experimentally infected with Strongyloides venezuelensis. The microtiter plates were coated with alkaline parasite extract for antibody detection and with IgG anti-S. venezuelensis for antigen and immune complex detection. The… CONTINUE READING