A novel approach allows identification of K channels in the lateral membrane of rat CCD.

@article{Wang1994ANA,
  title={A novel approach allows identification of K channels in the lateral membrane of rat CCD.},
  author={Wenhui Wang and Carmel M. McNicholas and Alan S Segal and Gerhard Giebisch},
  journal={The American journal of physiology},
  year={1994},
  volume={266 5 Pt 2},
  pages={F813-22}
}
We have developed a novel approach to study K channels in the lateral membrane of principal cells (PC) in rat cortical collecting ducts (CCD). The technique consists of 1) exposing the CCD apical membrane, 2) removing the intercalated cells adjoining a PC by gentle suction through a pipette, and 3) applying patch-clamp technique to the lateral membrane of PC. Functional viability of the PC was confirmed by three indexes: 1) maintenance of physiological cell membrane potentials (-85 +/- 3 mV); 2… CONTINUE READING